胡日查
教授，博士生导师
办公室：太阳集团tyc138420
电话：18047181136
邮箱：hbaigude@imu.edu.cn
教育背景
1987-1991：内蒙古师范大学生物系，获理学学士学位
1996-1999：ok138cn太阳集团生命科学学院攻读硕士学位，获理学硕士学位
2001-2004：日本帝京科学大学环境与材料科学系攻读博士学位，获理学博士学位
工作经历
1999-2001：太阳集团tyc138，讲师
2004-2005：日本北见工业大学，博士后研究员
2005-2008：美国University of Massachusetts Medical School, 博士后研究员
2008-2012：美国Sanford Burnham Prebys Medical Discovery Institute, 专职研究员
2012-至今：太阳集团tyc138，教授
学术兼职
内蒙古自治区蒙医药学会脑病专业委员会委员
中国民族医药学会脑病分会常务理事
学术期刊 BioMed Research International 学术编辑
学术期刊Discovery Chemistry学术编辑
荣誉与奖励
中共内蒙古自治区委员会组织部2013年、2018年“草原英才”奖
内蒙古自治区蒙医药学会脑病专业委员会2018年学术年会论文一等奖
中国民族医药学会2019年科学技术奖二等奖
内蒙古自治区自然科学奖二等奖（2024年）
研究领域
1. 核酸药物的靶向递送；
2. 天然多糖的改性及其生物活性；
3. 蒙药有效成分及作用机制；
教学
主讲太阳集团tyc138大三《生物化学》
研究项目
1. 国家自然科学基金委员会，地区科学项目，32460239，抗肿瘤反式扩增mRNA治疗剂的精准靶向性递送研究，2025-01-2028-12，32万，在研，主持；
2. 国家自然科学基金委员会，地区科学项目，32160231，双配体功能化LNP载体的设计及其胶质细胞靶向siRNA递送研究，2022-01-2025-12，34万，已结题，主持；
3. 国家自然科学基金委员会，面上项目，21875124，基于凝胶多糖的多功能细胞支架的设计及其抗肿瘤免疫治疗活性的研究，2019-01-2022-12，65万，已结题，主持；
4. 国家自然科学基金委员会，面上项目，21375058，利用亲和光交联和纳米微粒检测microRNA靶基因的研究，2014-01-2017-12，80万，已结题，主持；
5. 国家自然科学基金委员会，地区科学项目，21364006，利用分支多肽型纳米微粒靶向性传送siRNA的研究，2014-01-2017-12，50万，已结题，主持；
6. 国家自然科学基金委员会，地区科学项目，81560568，降血脂活性天然产物荜茇宁特异性靶标的确定及其作用机理研究，2016-01-2019-12，42万，已结题，主持；
7. 内蒙古自治区自然科学基金项目，2021MS02009，基于多糖型纳米微球的靶向性siRNA药物载体的设计，2021-01-2023-12，7万，已结题，主持；
8. 内蒙古自治区科技计划项目，额尔顿乌日勒和萨乌日勒对神经再生相关基因表达的调控作用研究，2015-01-201-/12，主要参与人，已结题；
9. 内蒙古自治区自然科学基金项目，萨乌日勒优化方对神经细胞的保护作用机理研究，2017-01-2019-12，主要参与人，已结题；
代表性成果
发表SCI期刊学术论文60余篇，获国家授权发明专利5项；
论文：
1. Yu X, Hao X, Chen M, Baigude H. Dual-functional anti-SIRPα-cGAMP conjugate reprograms the tumor immune microenvironment and enhances antitumor immunity. J Med Chem. 2026, Accepted. (通讯作者)
2. Yu X, Hao X, Li H, Ma Z, Ma Z, Ji L, Yang Z, Wang J, Chen M, Baigude H. Modulation of Tumor Microenvironment via Antibody Mediated Precision Delivery of CpG to Myeloid Cell. J Med Chem. 2026 Feb 26;69(4):4900-4912. (通讯作者)
3. Yu X, Ma ZM, Ji F, Chen M, Baigude H. Preparation and in vitro functional validation of a novel antibody-siRNA-Exatecan conjugate (AREC). Int Immunopharmacol. 2025 Nov 27;168(Pt 2):115938. (通讯作者)
4. Bao M, Yang D, Qiburi Q, He M, Temuqile T, Baigude H. Antidiabetic effects of a polyherbal formulation of Nigella sativa, Origanum vulgare, and Malus asiatica in STZ-induced type 2 diabetic rats. J Funct Foods. 2025, 135, 107111.(通讯作者)
5. Wang R, Bai H, Yang D, Wuhanqimuge, Bai S, Xiao H, Baigude H, Gao N. Overexpression of BDNF by Astrocytes Targeted Delivery of mRNA Ameliorates Cognitive Impairment in Mouse Model of TBI. ACS Chem Neurosci. 2025 Sep 17;16(18):3465-3471. (通讯作者)
6. Yang D, Chen S, Borijihan H, Aoqier A, Sarula S, Siqin S, Manda M, Temuqile T, Baigude H. Mechanism of Mongolian Medicine Batri-7 on Salmonella Enteritis. J Inflamm Res. 2025 Feb 3;18:1523-1541. (通讯作者)
7. Wang R, Zhu W, Bai N, Li M, Saqirila S, Bai H, Xiao H, Baigude H, Gao N. Curdlan-Mediated Syngeneic RNAi against NF-κB in Glial Cells Protects Cerebral Vessels in the TBI Mouse Model. Biomacromolecules. 2024 Oct 14;25(10):6780-6790. (通讯作者)
8. Xiao H, Bao X, Bai N, Zhu W, Saqirila S, Hu X, Bao Q, Baigude H. Synthesis of Lipidated Ligands and Formulation of Glia-Specific LNPs for RNAi-Mediated BBB Protection. J Med Chem. 2024 Aug 8;67(15):13217-13230. (通讯作者)
9. Bao Q, Bao M, Xiao H, Ganbold T, Han S, Baigude H. Tumor-Targeted Codelivery of CpG and siRNA by a Dual-Ligand-Functionalized Curdlan Nanoparticle. Biomacromolecules. 2024 Jun 10;25(6):3360-3372. (通讯作者)
10. Xiao H, Amarsaikhan O, Zhao Y, Yu X, Hu X, Han S, Chaolumen C, Baigude H. Astrocyte-targeted siRNA delivery by adenosine-functionalized LNP in mouse TBI model, Molecular Therapy-Nucleic Acids, Volume 34, 2023, 102065, ISSN 2162-2531, doi: 10.1016/j.omtn.2023.102065. (通讯作者)
11. Bao Q, Ganbold T, Bao M, Xiao H, Han S, Baigude H. Tumor targeted siRNA delivery by adenosine receptor-specific curdlan nanoparticles. Int J Biol Macromol. 2023 Sep 11; 253(Pt 3):126845. doi: 10.1016/j.ijbiomac.2023.126845. (通讯作者)
12. Sereejav E, Sandagdorj A, Bazarjav P, et al. Antibody responses to mRNA versus non-mRNA COVID vaccines among the Mongolian population. IJID Reg. 2023 Sep; 8:1-8. doi:10.1016/j.ijregi.2023.05.002.
13. Zhu D, Mengyue M, Qimuge A, Bilige B, Baiyin T, Temuqile T, Chen S, Borjigen S, Baigude H, Yang D. Oral Delivery of SARS-CoV-2 DNA Vaccines Using Attenuated Salmonella typhimurium as a Carrier in Rat. Mol Gen Microbiol Virol. 2022; 37(3):159-166. doi: 10.3103/S0891416822030107. (共同通讯作者)
14. Qiburi Q, Ganbold T, Aoqier A, Yang D, Su Z, Bao M, He M, Gaowa S, Temuqile T, Baigude H. Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia. J Chromatogr B Analyt Technol Biomed Life Sci. 2022, 1211:123458. doi: 10.1016/j.jchromb.2022.123458. (共同通讯作者)
15. Yu X, Xiao H, Muqier M, Han S, Baigude H. Effect of the array of amines on the transfection efficiency of cationic peptidomimetic lipid molecules into neural cells. RSC Adv. 2022,1 2(33):21567-21573. doi: 10.1039/d2ra03347j. (通讯作者)
16. Bo S, Dan M, Han W, Ochir S, Bao L, Liu L, Muschin T, Baigude H. Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from Mirabilis himalaica (Edgew) Heim. RSC Adv. 2022, 12(27):17264-17275. doi: 10.1039/d2ra00060a. (共同通讯作者)
17. Ganbold T, Bao Q, Xiao H, Zurgaanjin D, Liu C, Han S, Hasi A, Baigude H. Peptidomimetic Lipid-Nanoparticle-Mediated Knockdown of TLR4 in CNS Protects against Cerebral Ischemia/Reperfusion Injury in Mice. Nanomaterials (Basel). 2022, 12(12):2072. doi: 10.3390/nano12122072. (通讯作者)
18. Liao C, Bao M, Hasi Q, Chen Z, Qian J, Baigude H. The study of ethanol extract of Epilobium angustifolium L. on blood sugar level in type II diabetic rats. Pak J Pharm Sci. 2022, 35(2):425-433. PMID: 35642396. (通讯作者)
19. Xiao H, Han S, Baigude H. Regulation of microglia polarization via mannose receptor-mediated delivery of siRNA by ligand-functionalized DoGo LNP. RSC Adv. 2021, 11(52):32549-32558. doi: 10.1039/d1ra04293a. (通讯作者)
20. Bao Q, Ganbold T, Qiburi Q, Bao M, Han S, Baigude H, AMP functionalized curdlan nanoparticles as a siRNA carrier: Synthesis, characterization and targeted delivery via adenosine A2B receptor, Int. J. Biol. Macromol., 2021, 193, 866-873, doi: 10.1016/j.ijbiomac.2021.10.138. (通讯作者)
21. Muqier M, Xiao H, Yu X, Li Y, Bao Q, Han S, Baigude H. Synthesis of PEGylated cationic curdlan derivatives with enhanced biocompatibility. J Biomater Sci Polym Ed. 2022 33(4):465-480, doi: 10.1080/09205063.2021.1992589. (通讯作者)
22. Hai Xiao, Han S, Baigude H. Regulation of microglia polarization via mannose receptor-mediated delivery of siRNA by ligand-functionalized DoGo LNP. RSC Adv., 2021, 11, 32549. doi: 10.1039/d1ra04293a (通讯作者)
23. Bao M, Ehexige E, Xu J, Ganbold T, Han S, Baigude H. Oxidized curdlan activates dendritic cells and enhances antitumor immunity. Carbohydr Polym. 2021, 264:117988. doi: 10.1016/j.carbpol.2021.117988. (通讯作者)
24. Qiburi Q, Temuqile T, Baigude H. Synergistic Regulation of Microglia Gene Expression by Natural Molecules in Herbal Medicine. Evidence-Based Complementary and Alternative Medicine. 2021: 9920364. doi.org/10.1155/2021/9920364 (共同通讯作者)
25. Liao C, Han Y, Chen Z, Baigude H. The extract of black cumin, licorice, anise, and black tea alleviates OVA-induced allergic rhinitis in mouse via balancing activity of helper T cells in lung. Allergy Asthma Clin Immunol. 2021 Sep 7;17(1):87. doi: 10.1186/s13223-021-00587-6. PMID: 34493326; PMCID: PMC8424864. (通讯作者)
26. Su Z, Ganbold T, Baigude H. Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes. Int J Mol Sci. 2020 Feb 24;21(4):1545. doi: 10.3390/ijms21041545. (通讯作者)
27. Ganbold T, Bao Q, Zandan J, Hasi A, Baigude H. Modulation of Microglia Polarization through Silencing of NF-κB p65 by Functionalized Curdlan Nanoparticle-Mediated RNAi. ACS Appl Mater Interfaces. 2020 Mar 11;12(10):11363-11374. doi: 10.1021/acsami. 9b23004. (通讯作者)
28. Ehexige E, Bao M, Bazarjav P, Yu X, Xiao H, Han S, Baigude H. Silencing of STAT3 via Peptidomimetic LNP-Mediated Systemic Delivery of RNAi Downregulates PD-L1 and Inhibits Melanoma Growth. Biomolecules. 2020 Feb 12;10(2):285. doi: 10.3390/ biom10020285. (通讯作者)
29. Erdene-Ochir T, Ganbold T, Zandan J, Han S, Borjihan G, Baigude H. Alkylation enhances biocompatibility and siRNA delivery efficiency of cationic curdlan nanoparticles. Int J Biol Macromol. 2020 Jan 15;143:118-125. doi: 10.1016/ j.ijbiomac.2019.12.048. (通讯作者)
30. Su Z, Erdene-Ochir T, Ganbold T, Baigude H. Design of curdlan-based pH-sensitive polymers with endosome buffering functionality for siRNA delivery. Int J Biol Macromol. 2020 Mar 1;146:773-780. doi: 10.1016/j.ijbiomac.2019.10.129. (通讯作者)
31. Qiburi Q, Ganbold T, Bao Q, Da M, Aoqier A, Temuqile T, Baigude H. Bioactive components of ethnomedicine Eerdun Wurile regulate the transcription of pro-inflammatory cytokines in microglia. J Ethnopharmacol. 2020 Jan 10;246:112241. doi: 10.1016/j.jep.2019.112241. (通讯作者)
32. Ehexige E, Ganbold T, Yu X, Han S, Baigude H. Design of Peptidomimetic Functionalized Cholesterol Based Lipid Nanoparticles for Efficient Delivery of Therapeutic Nucleic Acids. Molecules. 2019 Sep 19;24(18). pii: E3413. doi: 10.3390/molecules24183413. (通讯作者)
33. Wang X, Qi Y, Liu L, Ganbold T, Baigude H, Han J. Preparation and cell activities of lactosylated curdlan-triornithine nanoparticles for enhanced DNA/siRNA delivery in hepatoma cells. Carbohydr Polym. 2019 Dec 1;225:115252. doi: 10.1016/j.carbpol. 2019.115252. Epub 2019 Aug 27. (通讯作者)
34. Ganbold T, Han S, Hasi A, Baigude H. Receptor-mediated delivery of therapeutic RNA by peptide functionalized curdlan nanoparticles. Int J Biol Macromol. 2019 Apr 1;126:633-640. doi: 10.1016/j.ijbiomac.2018.12.152. Epub 2018 Dec 18. (通讯作者)
35. Chen X, Tian F, Li M, Xu H, Cai M, Li Q, Zuo X, Wang H, Shi X, Fan C, Baigude H, Shan Y. Size-Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures. Glob Chall. 2019 Nov 20;4(3):1900075. doi: 10.1002/gch2.201900075.
36. Ganbold T, Baigude H. Design of Mannose-Functionalized Curdlan Nanoparticles for Macrophage-Targeted siRNA Delivery. ACS Appl Mater Interfaces. 2018, 10(17):14463-14474. (通讯作者)
37. Han S, Ganbold T, Bao Q, Yoshida T, Baigude H. Sugar Functionalized Synergistic Dendrimers for Biocompatible Delivery of Nucleic Acid Therapeutics. Polymers. 2018, 10(9), 1034 (通讯作者)
38. Gaowa S, Bao N, Da M, Qiburi Q, Ganbold T, Chen L, Altangerel A, Temuqile T, Baigude H. Traditional Mongolian medicine Eerdun Wurile improves stroke recovery through regulation of gene expression in rat brain. J Ethnopharmacol. 2018, 222:249-260. (通讯作者)
39. Dong J, Hu J, Baigude H, Zhang H. A novel ferrocenyl-naphthalimide as a multichannel probe for the detection of Cu(ii) and Hg(ii) in aqueous media and living cells. Dalton Trans. 2018 Jan 2;47(2):314-322. doi: 10.1039/c7dt03982d.
40. Gao X, Hai X, Baigude H, Guan W, Liu Z. Fabrication of functional hollow microspheres constructed from MOF shells: Promising drug delivery systems with high loading capacity and targeted transport. Sci Rep. 2016 Nov 23;6:37705. doi: 10.1038/srep37705.
41. Gerile G, Ganbold T, Li Y, Baigude H. Head group configuration increases biocompatibility of cationic lipids for nucleic acids delivery, Journal of Materials Chemistry B, 2017, 5: 5597-5607. (通讯作者)
42. Tong Y, Ganbold T, Baigude H. Synthesis of Amphoteric Curdlan Derivatives for Delivery of Therapeutic Nucleic Acids, Carbohydr Polym. 2017, 175: 739-745. (通讯作者)
43. Han J, Wang X, Liu L, Li D, Suyaola S, Wang T, Baigude H. "Click" chemistry mediated construction of cationic curdlan nanocarriers for efficient gene delivery. Carbohydr Polym. 2017, 163:191-198. (通讯作者)
44. Ganbold T, Gerile G, Xiao H, Baigude H. Efficient in vivo siRNA delivery by stabilized D-peptide-based lipid nanoparticles. RSC Adv., 2017, 7, 8823-8831. (通讯作者)
45. Hou X, Ganbold T, Baigude H. Synthesis of biocompatible amino acid-modified poly(acrylic acid) derivatives for intracellular gene delivery, International Journal of Polymeric Materials and Polymeric Biomaterials, 2017, 67:3, 174-180 (通讯作者)
46. Altangerel A, Cai J, Liu L, Wu Y, Baigude H, Han J. PEGylation of 6-amino-6-deoxy curdlan for efficient in vivo siRNA delivery. Carbohydr Polym. 2016, 141:92-8. (通讯作者)
47. Xiao H, Altangerel A, Gerile G, Wu Y, Baigude H. Design of Highly Potent Lipid-Functionalized Peptidomimetics for Efficient in Vivo siRNA Delivery. ACS Appl Mater Interfaces. 2016, 8(12):7638-45. (通讯作者)
48. Wu Y, Cai J, Han J, Baigude H. Cell Type-Specific Delivery of RNAi by Ligand-Functionalized Curdlan Nanoparticles: Balancing the Receptor Mediation and the Charge Motivation. ACS Appl Mater Interfaces. 2015, 7(38):21521-8.（通讯作者）
49. Han J, Cai J, Borjihan W, Ganbold T, Rana TM, Baigude H. Preparation of Novel Curdlan Nanoparticles for Intracellular siRNA Delivery. Carbohydr Polym., 2015, 117, 324–330.（通讯作者）
50. Yu S, Gao X, Baigude H, Hai X, Zhang R, Gao X, Shen B, Li Z, Tan Z, Su H. Inorganic nanovehicle for potential targeted drug delivery to tumor cells, tumor optical imaging. ACS Appl Mater Interfaces. 2015, 7(9):5089-96.
51. McCarroll JA, Dwarte T, Baigude H, Dang J, Yang L, Erlich RB, Kimpton K, Teo J, Sagnella SM, Akerfeldt MC, Liu J, Phillips PA, Rana TM, Kavallaris M. Therapeutic targeting of polo-like kinase 1 using RNA-interfering nanoparticles (iNOPs) for the treatment of non-small cell lung cancer. Oncotarget. 2014, 6(14):12020-34.
52. Baigude H, Rana TM. Strategies to antagonize miRNA functions in vitro and in vivo. Nanomedicine (Lond), 2014, 9(16), 2545-55. (第一作者)
53. Sarnaizul E, Borjihan G, Baigude H et al. LC analysis and pharmacokinetic study of synthetic piperlonguminine in rat plasma after oral administration. Biomed Chromatogr. 2013, 27(7), 821-4.
54. Baigude, H., Jie Su, Joshua McCarroll, and Tariq M. Rana. In Vivo Delivery of RNAi by Reducible Interfering Nanoparticles (iNOPs). ACS Med. Chem. Lett., 2013, DOI: 10.1021/ml4001003.
55. Baigude, H., Ahsanullah, Zhonghan Li, Ying Zhou, and Tariq Rana. miR-TRAP: a Benchtop Chemical Biology Strategy to Identify microRNA Targets. Angew. Chem. Int. Ed. 2012, 51, 5880 -5883.
56. Baigude, H., Tariq Rana. Interfering nanoparticles for microRNA delivery. Methods in Enzymology. 2012, 509, 339-53.
57. Baigude, H.*, Joshua McCarroll*, Chao-shun Yang, Tariq M. Rana. Nanotubes Functionalized with Lipids and Natural Amino Acid Dendrimers: A New Strategy to Create Nanomaterials for Delivering Systemic RNAi. Bioconjugate Chem. 2010, 21, 56-63.
58. Baigude, H., Tariq M. Rana. Delivery of Therapeutic RNAi by Nanovehicles. ChemBioChem 2009, 10, 2449-2454.
59. Baigude, H., Joshua McCarroll, Chao-shun Yang, Pamela M. Swain, and Tariq M. Rana. Design and Creation of New Nanomaterials for Therapeutic RNAi. ACS Chemical Biology, 2007, 2(4), 237-241.
60. Baigude, H., Kaname Katsuraya, Shinichi Tokunaga, Naohiko Fujiwara, Masaya Satoyama, Tomohiro Magome, Kohsaku Okuyama, Gereltu Borjihan, Toshiyuki Uryu. Synthesis of an oligosaccharide-polylysine dendrimer with reducing sugar terminals leading to acquired immunodeficiency syndrome vaccine preparation. J. Polym. Sci. A Polym. Chem., 2005, 43, 2195-2206.
61. Baigude, H., Kaname Katsuraya, Kohsaku Okuyama, Naoki Kariya, and Toshiyuki Uryu. Synthesis of HIV Vaccine Model Based on Lactose-Functionalized Poly(lysine) Dendrimer Scaffold. Sen’i Gakkaishi, 2004, 60, 118-124.
62. Baigude, H., Kaname Katsuraya, Kohsaku Okuyama, Kenichi, Hatanaka, Emi Ikeda, Naokazu Shibata, and Toshiyuki Uryu. Synthesis of Spherical and Hemispherical Sugar-Containing Poly(ornithine) Dendrimers. J. Polym. Sci. A Polym. Chem., 2004, 42, 1400-1414.
63. Baigude, H., Kaname Katsuraya, Kohsaku Okuyama, and Toshiyuki Uryu. Synthesis of Structurally-Controlled HIV Vaccine Model Having Glyco-Peptide Dendrimer Scaffolds” Macromol. Chem. Phys., 2004, 205, 684-691.
64. Baigude, H., Kaname Katsuraya, Kohsaku Okuyama, Shinichi Tokunaga, and Toshiyuki Uryu. Synthesis of Sphere-Type Monodispersed Oligosaccharide Polypeptide Dendrimers. Macromolecules, 2003, 36, 7100-7106.
65. Baigude, H., Kaname Katsuraya, Kohsaku Okuyama, Yoshihide Yachi, Shoma Sato, and Toshiyuki Uryu. Synthesis of Dicarboxylate Oligosaccharide Multilayer Terminal Functionality upon Poly(lysine) Dendrimer Scaffolding. J. Polym. Sci. A Polym. Chem., 2002, 40, 3622-3633.
66. Jie Su, Baigude, H., Joshua McCarroll and Tariq M. Rana. Silencing microRNA by interfering nanoparticles in mice. Nucleic Acids Research, 2011, 39(6), e38, doi:10.1093/nar/gkq1307.
67. Animesh Ghosh, Koushik Mukherjee, Xinpeng Jiang, Ying Zhou, Joshua McCarroll, James Qu, Pamela M. Swain, Baigude, H., and Tariq M. Rana. Design and Assembly of New Nonviral RNAi Delivery Agents by Microwave-Assisted Quaternization (MAQ) of Tertiary Amines. Bioconjugate Chem. 2010, 21 (9), 1581–1587.
68. Hongyan Wang, Animesh Ghosh, Baigude, H., Chao-shun Yang, Linghua Qiu, Xugang Xia, Hongxia Zhou, Tariq M. Rana and Zuoshang Xu. Therapeutic Gene Silencing Delivered by A Chemically Modified siRNA Against Mutant SOD1 Slows ALS Progression J. Biol. Chem., 2008, 283, 15845-15852.
专利：
1. 胡日查，海小，“一种阳离子脂质类化合物及其制备方法与应用”，专利号：ZL201510228936.4
2. 胡日查，海小，“一种含有二肽类脂质阳离子的化合物及其制备方法与应用”，专利号：ZL 201510228170.X
3. 胡日查，鲍明明，“一种基于黑种草的降血糖组合物以及降血糖蒙药”，专利号：ZL 201711071958.X
4. 胡日查，清明，“一种阳离子多糖及其制备方法与应用”，专利号：ZL 202110263793.6
5. 胡日查，海小，“一种单糖配体功能化的阳离子脂质类化合物及其制备方法与应用”，专利号：ZL 202110553801.0
